✦ LIBER ✦

Fluorescence microscopy of etched methacrylate sections improves the study of mitosis in plant cells

✍ Scribed by Hoffman, John C.; Vaughn, Kevin C.; Mullins, J. Michael

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 902 KB
Volume: 40
Category: Article
ISSN: 1059-910X
DOI: 10.1002/(sici)1097-0029(19980301)40:5<369::aid-jemt4>3.0.co;2-m

No coin nor oath required. For personal study only.

✦ Synopsis

for immunofluorescence microscopic investigation of the plant cytoskeleton, especially during mitosis. These advantages include: (1) unimpeded access of antibody probes, (2) confocal-like imaging without the expense of confocal equipment, (3) maintenance of organ architecture as well as intracellular structure, (4) the ability to independently examine separate focal planes with the same or multiple antibody(s) or other labelling compounds, and ( ) the ability to archive unetched sections, polymerized or non-polymerized infiltrated tissue. In this paper examples of staining of various microtubule cytoskeletal and mitotic proteins are shown in a variety of methacrylate embedded plant tissues.

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