Fluorescence lifetime distributions of parinaroyl phospholipids in choline plasmalogen and phosphatidylcholine bilayers containing different amounts of cholesterol

✍ Scribed by Josef Loidl; Fritz Paltauf; Albin Hermetter

• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 647 KB
• Volume: 56
• Category: Article
• ISSN: 0009-3084
• DOI: 10.1016/0009-3084(90)90085-6

✦ Synopsis

The fluorescence decay of alkenylparinaroyl-and palmitoylparinaroyl glycerophosphocholines in vesicles of the unlabeled alkenyloleoyl and palmitoyloleoyl analogs was determined by multifrequency phase and modulation fluorometry. The measured phase angles and demodulations could be equally well fitted to a biexponential decay, as well as unimodal or bimodal continuous lifetime distributions. The latter model was applied to study the influence of cholesterol on parinaroyl phospholipid fluorescence in vesicles. The long-riving component of a bimodal lifetime distribution was sensitive toward the presence of the sterol. Upon increasing cholesterol concentrations, its lifetime center increased and its distribution widths decreased. Lifetime distribution widths in vesicles of alkenyloleoyl-or palmitoyloleoyl-glycerophosphocholine (choline plasmalogen and phosphatidylcholine, respectively) were reduced by the sterol to the same extent. We interprete the sterol-induced lifetime distribution narrowing as an effect due to an increase of membrane homogeneity in cholesterol-phospholipid membranes.