Lifetime-Based Fluorescence Energy Trans
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R.B. Thompson; M.W. Patchan
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Article
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1995
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Elsevier Science
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English
β 554 KB
A new type of fluorescence transduction method for determining zinc in solution is described. The approach is based upon energy transfer from a fluorescent label on an enzyme, human carbonic anhydrase II, to a colored inhibitor which binds to zinc in the enzyme active site. If zinc is present in sol