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Fluorescence lifetime-based biosensing of zinc: Origin of the broad dynamic range

✍ Scribed by Richard B. Thompson; Marcia W. Patchan


Book ID
104912340
Publisher
Springer
Year
1995
Tongue
English
Weight
789 KB
Volume
5
Category
Article
ISSN
1053-0509

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Lifetime-Based Fluorescence Energy Trans
✍ R.B. Thompson; M.W. Patchan πŸ“‚ Article πŸ“… 1995 πŸ› Elsevier Science 🌐 English βš– 554 KB

A new type of fluorescence transduction method for determining zinc in solution is described. The approach is based upon energy transfer from a fluorescent label on an enzyme, human carbonic anhydrase II, to a colored inhibitor which binds to zinc in the enzyme active site. If zinc is present in sol