Fluorescence intensity decays of cyclohexane and methylcyclohexane with two-photon excitation from a high repetition rate frequency-doubled dye laser
✍ Scribed by Joseph R. Lakowicz; Ignacy Gryczynski
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 508 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1075-4261
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✦ Synopsis
We observed fluorescence emission from cyclohexane (CH) and methylcyclohexane (MCH) using picosecond pulses a t 298-300 nm from a frequency-doubled cavity-dumped R6G dye laser. The emission maxima for C H and MCH are 209 and 215 nm, respectively, which agree with previous studies using vacuum ultra-violet (VUV) excitation near 150 nm. The fluorescence intensities depended quadratically on peak laser intensity indicating two-photon excitation. The intensity decays resulting from two-photon excitation were measured using frequency-domain fluorometry and were found to be nearly singly exponential ranging from 0.59 to 0.80 ns. Two-photon induced fluorescence of cyclic and linear alkanes may provide new intrinsic spectroscopic probes of biological molecules.