Background: Spectral interference (overlap) from phagocytosed green-yellow (GY) microspheres in the flow cytometric, red fluorescence emission measurement channel causes errors in quantifying damaged/dead alveolar macrophages by uptake of propidium iodide. Methods: Particle burdens of uniform GY flu
โฆ LIBER โฆ
Fluorescence instability of propidium iodide-labelled microspheres in (para)formaldehyde
โ Scribed by Edgar L. Rohr; Walter Kaffenberger
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 244 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0196-4763
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Apoptotic cells with frequent DNA strand breaks may be detected by tagging with directly or indirectly labeled nucleotides incorporated by the use of terminal deoxynucleotidyl transferase (TdT). Propidium iodide (PI) is typically added for the simultaneous assessment of DNA content. PI was found to