Fluorescence in situ hybridization mapping of breakpoints in pleomorphic adenomas with 8q12–13 abnormalities identifies a subgroup of tumors without PLAG1 involvement
✍ Scribed by Eva Röijer; Koen Kas; Margareta Behrendt; Wim Van de Ven; Göran Stenman
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 316 KB
- Volume
- 24
- Category
- Article
- ISSN
- 1045-2257
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✦ Synopsis
Recently, we identified the PLAG1 gene as the target gene in pleomorphic adenomas with chromosome abnormalities involving 8q12. The majority of breakpoints were shown to reside within the 5Ј noncoding region of the gene. We now report three pleomorphic adenomas with breakpoints located distal to PLAG1 in band 8q13. These tumors had the following chromosome 8 abnormalities: ins(8;12)(q12-13;q14q15), t(8;12)(q13;q15), and t(6;8)(p21.3-22;q13). Fluorescence in situ hybridization mapping of the chromosome 8 breakpoints revealed a yeast artificial chromosome clone spanning the breakpoints in two tumors. In none of the cases was PLAG1 activated and/or disrupted. Three candidate genes, N8, HMGIC, and HMGIY, were analyzed for rearrangements and/or abnormal expression by using reverse transcriptase-polymerase chain reaction, rapid amplification of 3Ј cDNA ends, and Northern blot analyses.