Fluorescence in situ hybridization deletion mapping at 4p16.3 in bladder cancer cell lines refines the localisation of the critical interval to 30 kb
✍ Scribed by Sandra M. Bell; Jian Zuo; Richard M. Myers; Margaret A. Knowles
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 881 KB
- Volume
- 17
- Category
- Article
- ISSN
- 1045-2257
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✦ Synopsis
An allelotype analysis of transitional cell carcinoma of the bladder identified loss of heterozygosity (LOH) on chromosome arm 4p in 22% of tumours. In a more detailed LOH study of 178 bladder carcinomas, a 750 kb common region of deletion was identified between the markers D4S43 and D4SI 27 just telomeric t o the Huntington disease locus. To refine this region of deletion at 4p 16.3, we have carried out detailed fluorescence in situ hybridisation (FISH) analysis of I 2 bladder cancer cell lines by using a chromosome 4 centromeric probe combined with a series of cosmid probes from contigs spanning the 750 kb region of deletion. A common 30 kb region of deletion was identified at 4~16.3 in over one-third of the bladder cancer cell lines analysed. The present study has refined the localisation of the critical region of deletion from 750 kb t o approximately 30 kb, providing a precise starting point for positional cloning of the gene@) involved in bladder cancer from within a very gene-rich region on chromosome band 4~16.3. This study demonstrates that FISH can be used for fine deletion mapping of potential tumour suppressor gene regions. The utilisation of FISH analysis t o map chromosomal deletions should facilitate positional cloning of other genes as bacterial artificial chromosome (BAC) and yeast artificial chromosome (YAC) contigs of the human genome are established.