Fluorescence identification of zoochlorellae: A rapid method for investigating alga-invertebrate symbioses

✍ Scribed by Williamson, Craig E.

• Book ID: 102892491
• Publisher: John Wiley and Sons
• Year: 1977
• Tongue: English
• Weight: 666 KB
• Volume: 202
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1402020207

✦ Synopsis

Abstract

Using fluorescence microscopy I have developed a technique for the rapid identification and enumeration of zoochlorellae which does not require sectioning. Chlorophyll in algal symbionts fluoresces bright red when excited by ultra‐violet light. In a test of this system using green and white Spongilla lacustris I found a ratio of 42:1 (green:white) in the number of symbionts and a similar ratio of 36:1 in the amount of chlorophyll a and b present.