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Fluorescence Enhancement through Enzymatic Cleavage of Internally Quenched Dendritic Peptides: A Sensitive Assay for the AspN Endoproteinase

โœ Scribed by John M. Ellard; Thomas Zollitsch; W. Jon Cummins; Alan L. Hamilton; Mark Bradley


Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
130 KB
Volume
114
Category
Article
ISSN
0044-8249

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Fluorescence Enhancement through Enzymat
โœ John M. Ellard; Thomas Zollitsch; W. Jon Cummins; Alan L. Hamilton; Mark Bradley ๐Ÿ“‚ Article ๐Ÿ“… 2002 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 130 KB ๐Ÿ‘ 1 views

Fluorescence resonance energy transfer (FRET) systems in which a peptide sequence contains both a fluorophore and an internal quencher are amongst the best methods available for protease analysis and characterization. Numerous proteases have been studied using this method, including trypsin which cl