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Fluorescence energy transfer-labeled primers for high-performance forensic DNA profiling

✍ Scribed by Stephanie H. I. Yeung; Tae Seok Seo; Cecelia A. Crouse; Susan A. Greenspoon; Thomas N. Chiesl; Jeff D. Ban; Richard A. Mathies


Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
559 KB
Volume
29
Category
Article
ISSN
0173-0835

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We have designed, synthesized, and characterized fluorescent cyanine heterodimers that exploit resonance energy transfer to achieve strong emission above 650 nm with 488-nm excitation. Thiazole orange serves as the common fluorescence donor in these dyes and thiazole-indolenine, thiazole blue, or sy