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Fluorescence energy transfer in one dimension: Frequency-domain fluorescence study of DNA–fluorophore complexes

✍ Scribed by Badri P. Maliwal; Józef Kuśba; Joseph R. Lakowicz

Publisher
Wiley (John Wiley & Sons)
Year
1995
Tongue
English
Weight
883 KB
Volume
35
Category
Article
ISSN
0006-3525

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We used time-dependent fluorescence energy transfer to determine the distribution of donorto-acceptor distances in native and denatured troponin I(Tn1). The single tryptophan residue (Trp 158) of TnI served as the donor (D), and the acceptor (A) was a labeled cysteine residue (Cys 133). The time-dep