Fluorescence detection with high time resolution: From optical microscopy to simultaneous force and fluorescence spectroscopy
✍ Scribed by Alexander Gaiduk; Ralf Kühnemuth; Suren Felekyan; Matthew Antonik; Wolfgang Becker; Volodymyr Kudryavtsev; Carl Sandhagen; Claus A.M. Seidel
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 955 KB
- Volume
- 70
- Category
- Article
- ISSN
- 1059-910X
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Picosecond time‐resolution fluorescence signal detection over many hours is possible using the time‐correlated single photon counting (TCSPC) technique. Advanced TCSPC with clock oscillator set by the pulsed laser and data analysis provides a tool to investigate processes in single molecules on time scale from picoseconds to seconds. Optical imaging techniques combined with TCSPC allow one to study the spatial distribution of fluorescence properties in solution and on a surface. Mechanical manipulation of a single macromolecule by means of an atomic‐force microscope makes it possible to detect fluorescence signal changes as a function of mechanical conformations of a fluorescent dye attached to a single DNA molecule. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.