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Fluorescence decay parameters of tryptophan in a homogeneous preparation of human hemoglobin

✍ Scribed by A.G. Szabo; K.J. Willis; D.T. Krajcarski; B. Alpert

Publisher: Elsevier Science
Year: 1989
Tongue: English
Weight: 480 KB
Volume: 163
Category: Article
ISSN: 0009-2614
DOI: 10.1016/0009-2614(89)85188-7

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✦ Synopsis

Nanosecond fluorescence lifetime components, observed in hemoglobin samples purified by standard procedures, are removed by a HPLC purification step. The tryptophyl fluorescence of extensively purified human hemoglobin is found to decay as a simple single exponential. The fluorescence lifetime of x 25 ps is relatively insensitive to protein conformational changes such as those resulting from different ligation states of the heme iron.

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