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Fluorescence correlation spectroscopy in vivo

✍ Scribed by J. Mütze; T. Ohrt; P. Schwille

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
978 KB
Volume
5
Category
Article
ISSN
1863-8880

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✦ Synopsis

Abstract

This review will focus on the application and potential of FCS and FCCS in vivo. Practical issues and sources of artifacts when performing measurements in living cells are discussed. Finally, several extensions to conventional FCS, such as multiphoton excitation, scanning FCS, Fluorescence Lifetime Correlation Spectroscopy, multiplexing FCS and recent approaches to reach smaller excitation volumes are reviewed

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