Fluorescence-activated flow cytometry in the hematology clinical laboratory

✍ Scribed by Laurence Corash; Margaret Rheinschmidt; Sandi Lieu; Patricia Meers; Elizabeth Brew

• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 567 KB
• Volume: 9
• Category: Article
• ISSN: 0196-4763
• DOI: 10.1002/cyto.990090813

✦ Synopsis

Recent improvements in fluorescence-activated flow cytometry instrumentation now make the introduction of this technology into the routine hematology clinical laboratory feasible. Successful adaption to the clinical laboratory requires that this instrumentation be used with techniques that are efficient and cost-effective. It is also important that this technology be adaptable to the performance of multiple assays in order to maximize utilization of this complex and expensive intrumentation. We have introduced fluorescence-activated flow cytometry into the routine hematology laboratory for the performance of two assays: detection of platelet-associated immunoglobulin (PAIg) and enumeration of reticulocytes. Our technique for the measurement of PAIg has recently been described in detail (Corash and Rheinschmidt, 1986). In this report, greater emphasis is placed on the fluorescent technique for reticulocyte enumeration, since this technique has not yet been widely adopted in the routine clinical laboratory.