We examined the different steps necessary for the enzymatic digestion of proteins in the polyacrylamide matrix after gel electrophoresis. As a result, we developed an improved method for obtaining peptides for internal sequence analysis from 1-2 micrograms of in-gel-digested proteins. The long washi
✦ LIBER ✦
Fluorescamine as a reagent for location of proteins after electrophoresis in starch gel or on paper
✍ Scribed by S.J. Tata; G.F.J. Moir
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 705 KB
- Volume
- 70
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Fluorescamine (Fluram, Roche) has been used as a reagent for locating proteins after electrophoresis in starch gel and on paper. Attractive features of the method are speed, sensitivity, and no destaining of gel or paper is required. The latex protein herein, which is particularly difficult to stain by conventional methods, was detected very readily in starch gel and on paper with Fluorescamine.
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🌐
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⚖ 940 KB