Fluconazole susceptibility testing of Candida species by flow cytometry
โ Scribed by Young Hee Joung; Hye Ryoun Kim; Mi Kyung Lee; Ae Ja Park
- Book ID
- 108174436
- Publisher
- Elsevier Science
- Year
- 2007
- Tongue
- English
- Weight
- 129 KB
- Volume
- 54
- Category
- Article
- ISSN
- 0163-4453
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We have developed an 8 hr flow cytometry (FCM) method for assessing susceptibility of yeasts to amphotericin B (AmpB). The method detects both highlevel and relative-resistance to the drug. Variables found to affect fluorescence of control and AmpB treated cells included pH, presence of glucose, inc
Objective. -The increasing incidence of severe fungal infections and the difficulties encountered with the existing antifungal susceptibility testing methods led us to develop a new technique based on flow cytometry, using the fluorochrome FUN -1 ยฎ . Materials and methods. -We tested the susceptibil
## Abstract A method for detection of the susceptibility of __Candida__ species to amphotericin B is described. The technique is a modification of a similar flow cytometric technique designed to detect antibiotic susceptibility of __Staphylococcus aureus.__ Membrane potential changes caused by the