Flow-through sensing device based on derivative synchronous fluorescence measurements for the multi-determination of B6 vitamers
✍ Scribed by Danhua Chen; M.D. Luque de Castro; M. Valcárcel
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 444 KB
- Volume
- 261
- Category
- Article
- ISSN
- 0003-2670
No coin nor oath required. For personal study only.
✦ Synopsis
A method for the determmatlon of pyridoxal, pyridoxal-5-phosphate and pyrldoxlc aad based on derivative synchronous fluorlmetrlc measurements IS proposed The method mvolves the formation of a fluorescent complex between the analytes and beryllium m ammonia buffers of different pH which make it possible to dlscnmmate between pyrldoxal and pyndoxal-5-phosphate as the fluorescent features of the two complexes are slmdar m a medium of pH 9 9, but very different at pH 7 9 The calibration range was 0 05-15 00 PM (two orders of magmtude lower than m the conventIona flow-inJection method, and with a relatwe standard deviation smaller than 4 5% m this range) The method was apphed to the analysis of serum samples with good results