Flow-through partial-filling affinity capillary electrophoresis using a crossreactive antibody for enantiomeric separations

Flow-through partial-filling affinity ca

Flow-through partial-filling affinity capillary electrophoresis can estimate binding constants of neutral ligands to receptors via a competitive assay technique

✍ John Kaddis; Erica Mito; Joseph Heintz; Alfredo Plazas; Frank A. Gomez 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 109 KB 👁 1 views

This work evaluates the use of a competitive binding assay using flow-through partial-filling affinity capillary electrophoresis (FTPFACE) to estimate binding constants of neutral ligands to a receptor. We demonstrate this technique using, as a model system, carbonic anhydrase B (CAB, EC 4.2.1.1) an