𝔖 Bobbio Scriptorium
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Flow sorting and cloning of 6-thioguanine-resistant cells

✍ Scribed by L. Eriksson; H. Amnéus; J. Grawé


Book ID
113251771
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
244 KB
Volume
216
Category
Article
ISSN
0165-1161

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✍ Mitchell S. Turker; Raymond J. Monnat; Ken-Ichiro Fukuchi; Patricia A. Johnston; 📂 Article 📅 1988 🏛 Springer 🌐 English ⚖ 842 KB

Thioguanine-resistant primary clones were grown from single cell suspensions obtained from dog and human kidneys by enzymatic digestion. In medium containing a relatively high concentration (lO#g/ml) of thioguanine, thioguanine-resistant primary clones arose from each source at frequencies ranging f