Flow microfluorometric studies of lectin binding to mammalian cells. I. general features

✍ Scribed by Paul M. Kraemer; Robert A. Tobey; Marvin A. Van Dilla

• Publisher: John Wiley and Sons
• Year: 1973
• Tongue: English
• Weight: 781 KB
• Volume: 81
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040810303

✦ Synopsis

Abstract

Flow microfluorometry has been used to quantitate cell‐surface binding of fluorescein‐conjugated lectins. Frequency distributions of total surface binding of Concanavalin A per cell were prepared for a variety of cultured cell populations, including established cell lines, virus‐transformed lines and non‐transformed parental lines. In the case of growing Chinese hamster cells (line CHO), much of the variability of Con A binding per cell could be related to variability of cell size. Experiments with cells synchronized by mitotic selection indicated that the modal surface density of binding sites was almost constant throughout the cell cycle. However, as indicated by inhibition of binding with α‐methyl mannopyranoside and by the effect of trypsin, the sites on each cell were heterogeneous in chemical structure and/or exposure. Agglutinability of virus‐transformed cell lines or trypsin‐treated parental lines was demonstrated but could not be correlated closely with binding.