Flow-injection spectrophotometric determination of amino acids based on an immobilised copper(II) -zincon system
✍ Scribed by L.Lahuerta Zamora; J.Martínez Calatayud
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 396 KB
- Volume
- 281
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
The flow-injection spectrophotometric determination of different amino acids was carried out by reaction with copper(B) ions entrapped in a polymeric material and filling a packed-bed reactor; the released copperW, complexed with the amino acid, reacted with zincon in a basic medium producing a blue colour that was monitored at 600 nm. The method was applied to determine the contents of different amino acids in pharmaceutical formulations. The calibration graph for glycine was linear over the range 0.5-U) pg ml-' with a relative standard deviation of 0.8% (n = 6) at 10 pg ml-' and a sample throughput of 108 h-'.