Flow-injection immunosensor for triazine herbicides using Eu(III) chelate label fluorescence detection
✍ Scribed by Monika Wortberg; Cornelia Middendorf; Andreas Katerkamp; Thomas Rump; Jörg Krause; Karl Cammann
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 1003 KB
- Volume
- 289
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
An immunochemical flow-injection system for the determination of triazine herbicides based on principles of immunoaffinity chromatography was developed. Triazine herbicide derivatives immobilized on oxirane acrylic beads serve as the affinity column. They are saturated with fluorescently labelled monoclonal anti-herbicide antibodies prior to the assay. The label used is the fluorescent E&II) chelate W8044-Eu. A fraction of the fluorescent antibodies is replaced when exposed to analyte and this fluorescence is detected in a postcolumn mode by means of a special laser-based fluorimeter. With the reusable affinity column a detection limit of 1 pg 1-l for the herbicide atrazine was obtained.