Flow dichroism of deoxyribonucleic acid solutions

✍ Scribed by Chong Sung Lee; Norman Davidson

• Book ID: 102761302
• Publisher: Wiley (John Wiley & Sons)
• Year: 1968
• Tongue: English
• Weight: 998 KB
• Volume: 6
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1968.360060408

✦ Synopsis

The flow dichroism of dilute DNA solutions (AZ60 = 0.1) has been studied in a Couette-type apparatus with the outer cylinder rotating and with the light path parallel to the cylinder axis. Shear gradienw in the range of 5-160 see.-' were studied. The DNA samples were whole, "half," and "quarter" molecules of T4 bacteriophage DNA, and linear and circular Xb2b.c DNA. For the linear molecules, the fractional flow dichroism is a linear function of molecular weight. DNA is about 1.8 that of the circular molecule. For a given DNA, the dichroism is an approximately linear function of shear gradient, but with a slight upward curvature