โ Scribed by Marion G. Macey BSc, PhD, FRCPath (auth.), Marion G. Macey PhD (eds.)
No coin nor oath required. For personal study only.
Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. Throughout Flow Cytometry: Principles and Applications, well-informed expert contributors present theoretical descriptions and practical protocols on this important and complex laboratory technique and its applications. Immunologists and Hematologists in the field of pathology, as well as biological researchers working with both human and animal models will appreciate the simple, clear-cut style in which principles and protocols in this volume are presented, and will refer to this book time and time again for clear and easy-to-follow protocols.
Front Matter....Pages i-ix
Principles of Flow Cytometry....Pages 1-15
Cell Preparation....Pages 17-58
Fluorochromes and Fluorescence....Pages 59-112
Quality Control in Flow Cytometry....Pages 113-131
Experimental Design, Data Analysis, and Fluorescence Quantitation....Pages 133-145
Apoptosis Detection by Flow Cytometry....Pages 147-163
DNA Analysis by Flow Cytometry....Pages 165-179
Immunological Studies of Human Cells....Pages 181-207
Calcium: Cytoplasmic, Mitochondria, Endoplasmic Reticulum, and Flux Measurements....Pages 209-217
Further Functional Studies....Pages 219-256
Cell Sorting by Flow Cytometry....Pages 257-276
Back Matter....Pages 277-290
Laboratory Medicine; Cell Biology
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It's a very good produc; very practical and motivating when reading. I only sugest improve the relation between text and some figures.
Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic tex
Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic tex
Flow cytometry is a powerful technique for the rapid analysis of single cells in a mixture. In microbiology, flow cytometry permits the reliable and rapid detection of single or multiple microbes and can provide information about their distribution within cell populations. Flow cytometry may also le