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Flow cytometry of X and Y chromosome-bearing sperm for DNA using an improved preparation method and staining with Hoechst 33342

โœ Scribed by Johnson, L. A. ;Flook, J. P. ;Look, M. V.

Book ID
111663253
Publisher
Wiley (John Wiley & Sons)
Year
1987
Weight
557 KB
Volume
17
Category
Article
ISSN
0148-7280

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Slit-scan flow cytometry for consistent
Slit-scan flow cytometry for consistent high resolution DNA analysis of X- and Y-chromosome bearing sperm
โœ Wim Rens; Glenn R. Welch; David W. Houck; Carel H. van Oven; Lawrence A. Johnson ๐Ÿ“‚ Article ๐Ÿ“… 1996 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 918 KB

This paper describes the application of slit-scan flow cytometry for accurate DNA analysis of X- and Y-chromosome bearing sperm. The introduction of the slit-scanning technique was initiated to improve the consistency in resolution of the X and Y population from donor to donor. An optimal resolution