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Flow cytometry of human reticulocytes based on RNA fluorescence

✍ Scribed by H. J. Tanke; I. A. B. Nieuwenhuis; G. J. M. Koper; J. C. M. Slats; J. S. Ploem

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
835 KB
Volume
1
Category
Article
ISSN
0196-4763

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✦ Synopsis

Abstract

A fluorescent staining procedure based on pyronin Y is described. The technique has been used to stain RNA in human reticulocytes for subsequent flow analysis and sorting. Histograms of fluorescence values of mature red blood cells and reticulocytes obtained by flow cytometry of stained blood samples, contain more information than offered by conventional methods for counting reticulocytes. Analysis of the fluorescence histograms provides new descriptive parameters of the reticulocyte population. The potential importance of this method in experimental and routine hematology is illustrated for a number of clinical cases.

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