Flow cytometry chamber with 4π light collection suitable for epifluorescence microscopes
✍ Scribed by James V. Watson
- Book ID
- 102816157
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 732 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
✦ Synopsis
A compact, solid, spherico-ellipsoidal chamber (SEC), which has approaching 4n ("all around") light collection, has been developed for flow cytometry. This was mounted onto the stage of a standard fluorescence photomicroscope, and the camera was replaced by a photomultiplier. Both components can be added or removed in minutes. The increased light collection efficiency of the SEC (about 85%) compared with about 4% from stan-dard chambers enabled a fluorescence microscope with a 50 W mercury vapour lamp to "double" as a flow cytometer. The system was tested with microbeads and cells stained for DNA with ethidium bromide, and results were comparable to those obtained with our laser-based ins trument.