✦ LIBER ✦

Flow cytometry analysis of platelet antibodies

✍ Scribed by Dr. Paul R. Finley; R. Jane Williams; Carla Fletcher

Publisher: John Wiley and Sons
Year: 1988
Tongue: English
Weight: 708 KB
Volume: 2
Category: Article
ISSN: 0887-8013
DOI: 10.1002/jcla.1860020413

No coin nor oath required. For personal study only.

✦ Synopsis

We have devised assays to detect both circulating alloantibodies to platelets (indirect assay) and platelet-association IgG and IgM (direct assay) using a flow cytometric technique. A variety of patients with immune thrombocytopenia were studied. Employment of a confocal lens in the flow cytometer increased the discrimination power of the instrument. Patients with autoimmune thrombocytopenia (idiopathic thrombocytic purpura [ITP], systemic Iupus erythematosus (SLE), lymphoma, leukemia, and drug-induced thrombocytope-nia showed asignificant increase in plateletassociated antibody. Circulating antibodies to platelets (alloantibodies) were demonstrated in cases of platelet refractoriness and neonatal isoimmune purpura. Day-today precision of the assays ranged from 3% to 6% (coefficient of variation). No interference was shown in the presence of hemoglobin (5 glL), triglycerides (10 glL), or polyclonal and monoclonal immunoglobulinemia (50 glL: IgG, IgA, IgM). The sensitivity of the direct assay was 500 attograrns of IgG or IgM platelet.

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