Flow Cytometric Screening of cDNA Expression Libraries for Fluorescent Proteins
β Scribed by Paul H. Bessette; Patrick S. Daugherty
- Book ID
- 109386947
- Publisher
- American Institute of Chemical Engineers
- Year
- 2004
- Tongue
- English
- Weight
- 168 KB
- Volume
- 20
- Category
- Article
- ISSN
- 8756-7938
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Successful cloning with cDNA expression libraries involves interaction of ligand molecules (probes) with expressed fusion proteins of interest. So far those ligands leading to successful results fall into three classes: (i) antibodies, (ii) protein ligands that interact with the protein of interest,
## Abstract ## Background Several staining protocols have been developed for flow cytometric analysis of bacterial viability. One promising method is dual staining with the LIVE/DEAD BacLight bacterial viability kit. In this procedure, cells are treated with two different DNAβbinding dyes (SYTO9 a