Flow cytometric multiparameter analysis of proliferating cell nuclear antigen/cyclin and Ki-67 antigen: A new view of the cell cycle
✍ Scribed by Göran Landberg; Eng M. Tan; Göran Roos
- Book ID
- 115892483
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 717 KB
- Volume
- 187
- Category
- Article
- ISSN
- 0014-4827
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The change of human nuclear antigen expression in proliferating cells recognized by a monoclonal antibody, Ki-67, during the cell cycle was investigated in HeLa S3 cells using a bivariate-flow-cytometric analysis. The antigen was immunocytochemically stained with FITC, and DNA was stained with propi
## BACKGROUND. The understanding of proliferation is a central issue in oncology. Several methods exist for the assessment of the growth fraction and cell-cycle time, but comparative studies that give the clinician advice about the most reliable use of new markers are few. The aim of the current s