Flow cytometric methods for DNA aneuploidy and cell cycle analysis
β Scribed by Larsen, J.K.; Christensen, I.J.
- Book ID
- 122496396
- Publisher
- Elsevier Science
- Year
- 1988
- Weight
- 118 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0277-5379
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## Abstract We describe an algorithm for fully automated flow cytometric DNA histogram classification and analysis that provides rapid, reproducible determination of DNA index and Sβphase fraction (SPF). Automated classification agreed with subjective assessment of DNA ploidy in 96β98% of DNA histo
DNA histograms were measured by flow cytometry for 656 human solid tumors (365 primary and 291 metastatic). The proportion of aneuploid cells in cell suspensions obtained by mechanical disaggregation was significantly higher than those obtained after enzymatic disaggregation (collagenase + DNAse) of