✦ LIBER ✦

Flow cytometric measurement of rates of particle uptake from dilute suspensions by a ciliated protozoan

✍ Scribed by Daniel P. Lavin; A. G. Fredrickson; Freidrich Srienc

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 854 KB
Volume: 11
Category: Article
ISSN: 0196-4763
DOI: 10.1002/cyto.990110804

No coin nor oath required. For personal study only.

✦ Synopsis

Flow cytometry is used to measure rates of ingestion of particles from dilute monodisperse suspensions by the ciliate Tetrahymena pyriformis. The particles used are polystyrene microspheres containing a fluorescent dye. Measurements were made directly, that is, by determining the fluorescence intensities from microspheres ingested by cells in samples collected from the experimental feeding apparatus. The fact that fluorescence intensities from individual cells can be grouped into discrete classes based on the numbers of fluorescent particles associated with the cells makes it possible to calibrate the flow cytometer and convert fluorescence measurements into numbers of particles ingested by average

📜 SIMILAR VOLUMES

Flow cytometric measurement of phagocyto

Flow cytometric measurement of phagocytosis reveals a role for C3b in metal particle uptake by phagocytes

✍ L. Baldwin; B. F. Flanagan; J. A. Hunt 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 135 KB

## Abstract A methodology for the quick and efficient study of phagocytosis has been developed. It uses the flow cytometer to exploit the change in size and granularity that occurs in cells upon the ingestion of particulate material. The numbers of cells that have phagocytosed particles can be calc