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Flow cytometric measurement of fluorescence (Förster) resonance energy transfer from cyan fluorescent protein to yellow fluorescent protein using single-laser excitation at 458 nm

✍ Scribed by Liusheng He; Thomas D. Bradrick; Tatiana S. Karpova; Xiaoli Wu; Michael H. Fox; Randy Fischer; James G. McNally; Jay R. Knutson; Amrie C. Grammer; Peter E. Lipsky

Publisher: John Wiley and Sons
Year: 2003
Tongue: English
Weight: 882 KB
Volume: 53A
Category: Article
ISSN: 0196-4763
DOI: 10.1002/cyto.a.10037

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Background: Development of spectrally distinct green fluorescent protein (GFP) variants has allowed for simultaneous flow cytometric detection of two different colored mutants expressed in a single cell. However, the dual-laser methods employed in such experiments are not widely applicable since the