Flow cytometric evaluation of nitro blue tetrazolium (NBT) reduction in human polymorphonuclear leukocytes

Differentiation of HL-60 promyelocytic l

Differentiation of HL-60 promyelocytic leukemia cells monitored by flow cytometric measurement of nitro blue tetrazolium (NBT) reduction

✍ Dr. Owen C. Blair; Rocco Carbone; Alan C. Sartorelli 📂 Article 📅 1985 🏛 John Wiley and Sons 🌐 English ⚖ 641 KB

Reduction of nitro blue tetrazolium (NBT) to insoluble blue formazan granules occurs during the stimulus-induced respiratory burst of mature granulocytes and is routinely used as a n indicator of the extent of granulocytic differentiation of HL-60 acute promyelocytic leukemia cells. In the present s