Flow cytometric enumeration of absolute lymphocyte number in peripheral blood using two parameters of light scatter
✍ Scribed by Dr. Y. C. Smart; J. Cox; B. Murphy; A. Enno; R. C. Burton
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 294 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
✦ Synopsis
A method was developed to measure the absolute lymphocyte count (ALC) of whole blood using the Spectrum 111 automated flow cytometer. Ninety-nine samples of human peripheral blood were analysed on the Spectrum and the Coulter Counter S Plus 11, to allow for comparison of the two machines. Regression analysis was used to test the extent of agreement between the sets of measurements on the two machines. The results demonstrated that the slope of the regression line was not significantly different from one, indicating a high level of correlation between Spectrum and Coulter ALC's. However, the mean difference between Coulter and Spectrum ALC's was not equal to zero, with the Spectrum giving counts approximately 10% lower than those of the Coulter machine. This is attributed to the different ways by which the two machines define a lymphocyte, the Spectrum 111 by two parameters of light scatter and the Coulter S Plus I1 by the single parameter of cell volume.