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Flow cytometric DNA content analysis in primary lung cancer: Comparison of results from fresh and paraffin-embedded specimens

โœ Scribed by Hiroshi Isobe; Hiroshi Miyamoto; Kazuaki Inoue; Mikio Shimizu; Takashi Endo; Shigetaka Mizuno; Yoshikazu Kawakami

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
334 KB
Volume
43
Category
Article
ISSN
0022-4790

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โœฆ Synopsis

Tumor DNA content was determined by flow cytometry in 24 surgically resected specimens from patients with primary lung cancer to compare results from fresh and paraffin-embedded specimens. We found a good correlation in DNA indices between fresh and paraffin-embedded specimens (I. = .978), although one tumor with DNA near-diploidy was mistaken as DNA diploid in paraffin-embedded specimens. In the measurements of paraffin-embedded specimens, eight of 24 tumors were DNA multiploid and showed DNA content heterogeneity. These results indicate that DNA content analysis of paraffin-embedded specimens is helpful because it makes it easy to analyze multiple specimens from different parts of the tumor.

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