✦ LIBER ✦

Flow cytometric determination of circulating immune complexes with the indirect granulocyte phagocytosis test

✍ Scribed by L. W. M. M. Terstappen; B. G. de Grooth; G. M. J. Nolten; C. H. H. ten Napel; W. van Berkel; J. Greve

Book ID: 102138345
Publisher: John Wiley and Sons
Year: 1985
Tongue: English
Weight: 496 KB
Volume: 6
Category: Article
ISSN: 0196-4763
DOI: 10.1002/cyto.990060407

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✦ Synopsis

A method for the determination of circulating immune complexes (CIC) was adapted for flow cytometric analysis. Human granulocytes were used to phagocytose I&-bearing CIC of serum from systemic lupus erythematosus (SLE) patients. A method for labeling the phagocytosed CIC with FITC-conjugated anti-human IgG was developed where the granulocytes remain in suspension during fixation and labeling. The fluorescence per cell, measured with a flow cytometer, is a measure of the total amount of the phagocytosed IgG.

The results indicate that a rapid and quantitative method for the detection and measurement of phagocytosed CIC is possible using the flow cytometer.

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