Flow cytometric determination of breast tumor heterogeneity
β Scribed by Joseph E. Fuhr; Ashley Frye; Anthony A. Kattine; Stuart Van Meter
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 489 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0008-543X
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A flow cytometric assay has been developed for determination of intracellular free potassium concentration ([K 1 ] i ). Investigated cells, loaded with the fluorescent pH indicator 28,7bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF), are incubated in the presence of nigericin, and the intra
Flow cytometric DNA analysis of isolated nuclei was performed on 14 lesions occurring in ten children with adrenocortical tumors. Unimodal DNA content distributions were obtained from seven tumors occurring in patients without metastases 2 to 18 years after diagnosis. Abnormal DNA contents were dete