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Flow cytometric detection of HLA antibodies using a spectrum of microbeads

✍ Scribed by Rui Pei; Jar-how Lee; Terrence Chen; Susan Rojo; Paul I. Terasaki

Book ID
117415951
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
334 KB
Volume
60
Category
Article
ISSN
0198-8859

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Intracellular glycosyltransferase protein expression can be assessed by flow cytometry. We report the detectability of the Golgi associated b1,4 galactosyltransferase (GT) and a2,6 sialyltransferase (ST) upon permeabilization in Jurkat and EBV-JY cells representing a T-and B-lymphoid cell line, resp