Flow cytometric analysis of variations in the DNA content of superficial and deep layers in colorectal cancer

✍ Scribed by Dr. Hirotsugu Tomoda; Yoshihiro Kakeji; Tetsuya Inoue

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 325 KB
• Volume: 57
• Category: Article
• ISSN: 0022-4790
• DOI: 10.1002/jso.2930570113

✦ Synopsis

We investigated variations in the DNA content of multiple samples from both superficial and deep layers of tumors from 81 colorectal cancer cases. The findings were only diploid in 21 cases (25.9%) and only aneuploid in 49 cases (60.5%). On the whole, the DNA ploidy agreed in 70 a s e s (86.4%) between both layers. In the 49 cases with only aneuploid tumors in both layers, the DNA index (mean k SD) was 1.58 * 0.25 in the superficial layer and 1.59 * 0.25 in the deep layer, with the values bcing almost identical. In contrast, a variation in the DNA ploidy of 1 I cases (13.6%) was observed. These findings show that DNA ploidy remains stable during tumor progression from the superficial into the deep layers in most cases of colorectal cancer. However, the existence of heterogeneity in the DNA ploidy also shows that several specimens should be collected from both layers before making a determination of DNA ploidy.