Flow cytometric analysis of the expression of 9A3 antigen, E-cadherin and EGF receptor in TMK-1 stomach cancer cells
✍ Scribed by Ryuichi Fukuyama; Shinsei Minoshima; Atsushi Ochiai; Eiichi Tahara; Nobuyoshi Shimizu
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- French
- Weight
- 812 KB
- Volume
- 48
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
We have previously produced a monoclonal antibody (MAb) against TMK-I cells, a poorly differentiated stomach-cancer cell line, and designated the antigen as 9A3. Immunohistochemical analysis of various tumor tissues revealed that the expression of 9A3 antigen is specific for human adenocarcinomz In the present study, we analyzed the expression patterns of PA3 antigen on TMK-I cells and compared them with the expression patterns of E-cadherin and epidermalgrowth-factor EGF) receptors, using immunofluorescent mirevealed that the TMK-I cell population consists of 9A3positive cells and 9A3-negative cells. The isolated 9A3negative cell population generates PA3-positive cells as they divide, whereas the isolated 9A3-positive cell population does not generate 9A3-negative cells. The 9Abpositive cells grow as tight colonies, whereas the 9A3-negative cells form loose colonies in soft agar culture. E-cadherin was expressed only in the OA3-positive TMK-I cells and not in the 9A3-negative cells. In contrast, EGF receptor was expressed in both cell populations. The 9A3 antigen was localized mainly at the cellcell adhesion sites where E-cadherin was apparently condensed. ~o ~c o p r and I ow cytometry. The flow cytometric analysis