Flow cytometric analysis of opposite effects of a monokine on proliferation and differentiation of murine B lymphocytes

✍ Scribed by Vongthip Souvannavong; Spencer C. Brown; Arlette Adam

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 709 KB
• Volume: 13
• Category: Article
• ISSN: 0196-4763
• DOI: 10.1002/cyto.990130510

✦ Synopsis

A 35,000 mw factor able to replace macrophages (FRM) in the induction of the in vitro antibody response to sheep erythrocytes has been isolated from the supernatant of murine resident peritoneal macrophage cultures. Purified FRM, when added at the outset of cultures, induced B cells to generate an antigen-specific antibody response. The signals provided by FRM in the process of B cell activation were analyzed using a polyclonal model. Cell cycle analysis by multiparameter flow cytometry after acridine orange staining showed that FRM, on its own, did not trigger the transition of B cells from the GO to the G1 stage of the cell cycle. In addition, FRM affected neither the basal intracellular free calcium level ([Ca2+li) nor the rapid increase in [Ca2+li induced by crosslinking of membrane immunoglobulin (mIgM) with anti-p antibodies. In parallel with its positive effect on B cell differentiation, FRM markedly reduced both proliferation and cell cycle progression of B cells stimulated with anti-p plus interleukin 4 (IL-4). Indeed, the addition of FRM to such cultures led to a preferential accumulation of cells in the early G1 compartment of the cell cycle and to a decreased frequency of cells in all other phases including GlB, S and G2/M.