Flow cytometric analysis of material-induced platelet activation in a canine model: Elevated microparticle levels and reduced platelet life span
✍ Scribed by Gemmell, Cynthia H. ;Yeo, Erik L. ;Sefton, Michael V.
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 83 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0021-9304
No coin nor oath required. For personal study only.
✦ Synopsis
Assessment of material-induced platelet activa-lead to elevated microparticle levels. MP levels were near tion is important given that it is thought to be a major mecha-background 24 h after removal of the PVA test segment. To nism of biomaterials thrombogenicity. We monitored, by determine platelet life span during the connection of test flow cytometry, platelet microparticle (MP) levels in the cir-segments, platelets were labeled in vivo with biotin and their culation during the connection of polyvinyl alcohol (PVA) disappearance monitored flow cytometrically. While platelet hydrogel and polyethylene (PE) test segments (3.18 mm ID, life span for shunted dogs (no test segment) was 4.7 Ϯ 0.2 20 and 50 cm L) to our chronically shunted beagle dogs. We days, the connection of PVA hydrogel test segments led to report that circulating microparticle levels were dependent a platelet life span of Ͻ2 days.