Flow cytometry offers the possibility to simultaneously analyze, on a cell by cell basis, different parameters related to cell viability i.e. cell size, morphology and incorporation of dyes. Different types of analysis: light absorption of unstained/stained cells, forward angle light scattering (FAL
Flow cytometric analysis of endothelial cell viability in arterial allograft
β Scribed by Chang-Young Lim; Eun Kyung Hong
- Publisher
- Springer
- Year
- 2011
- Tongue
- English
- Weight
- 504 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1061-1711
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Background: Combined analysis of DNA content and immunofluorescence on single cells by flow cytometry provides information on the proliferative response of subpopulations to stimuli in mixed cell preparations; however, in low-viability cell preparations, dead cells interfere with accurate flow cytom
Accurate estimation of cellular viability is important both in research and in aspects of orthopaedic clinical practice. We have been interested in the potential for flow cytometric application of fluorescein diacetate (FDA) in evaluating chondrocyte survival following cryopreservation of osteochond