✦ LIBER ✦
Flow cytometric analysis of cellular endogenous fluorescence simultaneously with emission from exogenous fluorochromes, light scatter and absorption
✍ Scribed by Bo Thorell
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- English
- Weight
- 392 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
A cytofluorimeter capable of simultaneous 4‐parameter analysis (i.e., endogenous fluorescence (NAD(P)H), exogenous fluorescent dyes, absorption and small angle scatter) is described. Analyses have been performed on isolated rat liver cells, white blood cells, bone marrow, thymus cells and yeast under different metabolic states. This allows correlations between cell function and concentration of cell substances, size, refractive indices, etc. Kinetic measurements are also possible.