Flourimetric determination of potassium ion using hexadecyl-acridine orange immobilized on a poly(vinylchloride) membrane attached to a flow-through cell
✍ Scribed by Yuji Kawabata; Totaro Imasama; Nobuhiko Ishibashi
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 399 KB
- Volume
- 255
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
Abslraet A catiomc fluorescent probe of hexadeeyl-acrldme orange 1s unmobdlzed on a poly(vmy1 chloride) membrane cnntammg vabnomycm The membrane is attached to the mner wall of the flow-through cell m a fluorescence spectrometer, and potassmm ion IS detected repetltwely by a flow mjectlon system wthout consummg the Indicator reagent The response IS proportlonal to the loganthmlc concentration of the potassium ion m the range 0 5-50 mM Ftieen samples are determmed 111 1 h, and the relative standard devlatlon of the peak he&t IS 2 4% for a sample contammg 0 1 pm01 (1 mM) of potassmm Ion The extended N~colsky equation 1s apphed to the observed peak height, the selectMty cocfficlent for sodmm IOII being 4 X 10m3 The concentration of potassmm ion m human blood plasma 1s also determmed by this system