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Flip and flop variants of AMPA receptor subunits in the human cerebellum: Implication for the selective vulnerability of purkinje cells

✍ Scribed by Masahiko Tomiyama; José M. Palacios; Roser Cortés;; Guadalupe Mengod


Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
410 KB
Volume
31
Category
Article
ISSN
0887-4476

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✦ Synopsis


We investigated the distribution of messenger RNAs coding for flip and flop splice variants ofAMPA receptor subunits in the human cerebellum to determine the relevance of AMPA receptors in the selective vulnerability of Purkinje cells to ischemia. Purkinje cells more abundantly expressed transcripts for flip variant of GluR-A, GluR-C, and GluR-D than granule cells, whereas transcripts for flop variants and GluR-B flip were expressed at similar levels on Purkinje cells and granule cells. These results suggest that human Purkinje cells possess AMPA receptors of the slowly desensitizing class as compared to granule cells. This differential distribution may explain the selective vulnerability of Purkinje cells.