FLIM on a Spectral Confocal Microscope : An Ultimate Tool for FRET Analysis

✍ Scribed by Maarten Balzar

• Publisher: Wiley (John Wiley & Sons)
• Year: 2006
• Weight: 272 KB
• Volume: 8
• Category: Article
• ISSN: 1439-4243
• DOI: 10.1002/imic.200790042

✦ Synopsis

The study of interactions between proteins is becoming increasingly important for live cell research. Since the fluorescent proteins have become available, live cell microscopy has become an important tool. It has been shown that fluorescence resonance energy transfer (FRET) can function as a tool to study protein interactions. In this application note, we focus on different methods to study fluorescence resonance energy transfer between cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP). Fast spectral detection and fluorescence lifetime imaging (FLIM) are competent and complementary methods for the study of interaction between proteins in living cells.