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Fixation and permanent mounting of fluorescent probes after vital labelling of cultured cells

✍ Scribed by Magdalena Cañete; Angeles Juarranz; Pilar López-Nieva; Carolina Alonso-Torcal; Angeles Villanueva; Juan C. Stockert

Book ID
117850662
Publisher
Elsevier Science
Year
2001
Tongue
German
Weight
266 KB
Volume
103
Category
Article
ISSN
0065-1281

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Use of fluorescent europium chelates as
Use of fluorescent europium chelates as labels in microscopy allows glutaraldehyde fixation and permanent mounting and leads to reduced autofluorescence and good long-term stability
✍ Lahja Sevéus; Mikko Väisälä; Ilkka Hemmilä; Hannu Kojola; Godfried M. Roomans; E 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 583 KB

In the present study europium chelates were introduced as alternative fluorescent labels for microscopy and their effect on enhanced autofluorescence caused by the glutaraldehyde fixative was investigated. Glutaraldehyde fixation was used to stabilize the cells for a permanent mount after the immuno